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Mouse Mammary Tumor Virus Envelope Protein Env antibody

The Rabbit Polyclonal anti- antibody has been validated for ELISA and WB. It is suitable to detect in samples from Mouse Mammary Tumor Virus (MMTV).
Catalog No. ABIN7565897

Quick Overview for Mouse Mammary Tumor Virus Envelope Protein Env antibody (ABIN7565897)

Target

Mouse Mammary Tumor Virus Envelope Protein Env

Reactivity

Mouse Mammary Tumor Virus (MMTV)

Host

Rabbit

Clonality

Polyclonal

Application

ELISA, Western Blotting (WB)
  • Purpose

    MMTV Antibody

    Characteristics

    Synonyms: rabbit anti-MMTV Antibody, rabbit anti-mouse mammary tumor virus surface protein antibody, Envelope glycoprotein gp70, Surface protein, Glycoprotein 52, Transmembrane protein, Glycoprotein 36, Mouse mammary tumor virus (strain GR) (MMTV)

    Purification

    Mouse mammary tumor virus surface protein antibody was prepared from monospecific, delipidated and defibrinated antiserum, with addition of sodium azide to 0.01 % and cross adsorbed with 6X HIS.

    Sterility

    Sterile filtered

    Immunogen

    Immunogen: MMTV was prepared from whole rabbit serum produced by repeated immunizations with a full length sequence for mouse mammary tumor virus surface protein tagged with His.

    Immunogen Type: Native Protein

  • Application Notes

    Application Note: Anti-MMTV Antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect band approximately 41.2 kDa in size corresponding to MMTV surface protein by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1:1000-1:2000 ELISA Dilution: 1:10,000-1:20,000

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    66 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

    Expiry Date

    12 months
  • Target

    Mouse Mammary Tumor Virus Envelope Protein Env

    Target Type

    Viral Protein

    Background

    Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI) and is suitable for Cancer, Enzyme, and Viral research. Mouse mammary tumor virus surface protein binds the virus to the receptor of the host cells triggering transmembrane protein refolding. This activates the fusion peptide and the structure coils and repeats positioning the fusion peptide near the C-terminus region. Inturn increasing close proximity and then fusion of viral and cell membranes. Anti-MMTV is ideal for researchers interested in Cancer, Viral, and Enzyme research.

    UniProt

    P03374
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